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KMID : 0604520060320010023
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Journal of the Society of Cosmetic Scientists of Korea
2006 Volume.32 No. 1 p.23 ~ p.28
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Separation and Purification of Effective Components from the Alisma orientale and its Application as a Cosmeceutical Ingredient
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Lee Dae-Woo
Kim Young-Jin
Kim Young-Sil
Kim Jong-Heon
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Abstract
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In this study, we performed anti-oxidation, whitening, cell recovery and anti-inflammation effects with Alisma orientale to evaluate the cosmeceutical properties. Alisma orientate extract (30, 70, 100 % MeOH) exhibited a significant tree radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and showed tyrosinase inhibition effect in a dose dependent manner (over 0.5% concentration). In cell proliferation assay using human fibroblast, it didn’’t show any proliferation effect but showed safety from cytotoxicity under 0.05% concentration. For whitening assay, we evaluated the melanin synthesis rate using B16 melanocyte and it showed a significant inhibitory effect (up to 40% under 0.05% concentration). After major screening assay, we separate 3 fractions from Alisma orientate extract by MPLC and performed cell recovery assay, melanin synthesis inhibition assay and anti-inflammatory assay. The third fraction showed a cell recovery effect over 30% against radical damage and remarkable repression in melanin synthesis and COX-2 synthesis.
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KEYWORD
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Alisma orientale, anti-oxidation, whitening, cell recovery, anti-inflammation
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